The insertion of Velocigene cassette ZEN-Ub1 created a deletion of size 7725bp between positions 61009749-61017473 of Chromosome 14 (Build GRCm39). A lacZ and floxed neomycin resistance cassette replaced exon 3. Western blot analysis confirmed absence of detectable protein in white adipose tissue lysates and serum from homozygous mutant mice.