Allele/Variant

Tg(Col2a1-rtTA,tetO-cre)22Pjro

Species
Mus musculus
Symbol
Tg(Col2a1-rtTA,tetO-cre)22Pjro
Category
allele
Allele of gene
None
Transgenic Constructs
Synonyms
  • COL2-rtTA-Cre
  • Tg(Col2a1-rtTA-tetO-cre)22Pjro
Description
The construct was generated such that a 6 kb fragment of the Col2a1 gene (composed of 3 kb of promoter and 3 kb of intron 1 containing an enhancer sequence) controls the reverse tetracycline transactivator sequence. A tetracycline responsive element heptad sequence (tetO) with a CMV minimal promoter is upstream of the cre recombinase coding sequence in this construct. Cre expression can only occur by transactivation of rtTA by doxycycline. The linearized construct was injected into fertilized oocytes and founder mice were obtained (lines 13, 12, 22, and 24). Cre mRNA expression was detected only in cartilage, not in the brain, liver or skin of embryos exposed to doxycycline.
Additional Information
Literature

Transgenic Constructs

This allele contains 1 transgenic construct
Symbol
Expressed Components
None
Knock-down Targets
None
Regulatory Regions
  • Col2a1
All alleles with this construct

Genomic Variant Information

No data available

Variant Molecular Consequences

No data available

Phenotypes

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    Disease Associations

    Association
    Disease Qualifier
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