The Minos-specific double-transposon trap inserted into intron 10 and intron 11. No fluorescent marker expression was detected in macrophages. The reduction in transcript expression was confirmed by RT-PCR on intraperitoneal cells. This allele was generated by crossing Hist1h1ttm1.1(mm)Ddra or Tg(Hist1h1t-mm)ADdra mice with TgTn(mm-DTT)1Ddra mice.