Version: 8.0.0Date: Tue Jan 28 2025
Allele/Variant
Tg(Tph2-cre/ERT2)1.38Zi
- Species
- Mus musculus
- Symbol
- Tg(Tph2-cre/ERT2)1.38Zi
- Category
- allele
- Allele of gene
- None
- Transgenic Constructs
- Synonyms
- 1.38
- TPH2-CreERT2
- Description
- A 177 kb PAC (P1-derived artificial chromosome; pPAC4) containing the full-length murine Tph2 gene and flanking sequences were modified by recombineering methods in EL250 bacteria prior to use for transgene construction. First, a chloramphenicol resistance gene replaced the kanamycin gene in the PAC backbone. Then a cassette encoding cre fused to a mutated human estrogen receptor (ER) ligand-binding domain (LBD; creERT2) and an FRT-flanked kanamycin resistance gene was integrated into the ATG start codon. A 23 bp sequence downstream of the ATG codon was deleted as it contained other in-frame ATG start sites in exon 1 of Tph2. Arabinose-induced FLP expression deleted the kanamycin cassette. The modified Tph2 sequence was excised from the PAC backbone and purified for injection into C57BL/6N oocytes. Five founder lines were obtained (1.21, 1.23, 1.27, 1.35, and 1.38). Lines 1.21 and 1.38 produced the highest recombination efficiency in serotonergic neurons with tamoxifen induction (91 and 90% respectively). Line 1.38 was investigated most extensively.
- Additional Information
- Literature
Transgenic Constructs
This allele contains 1 transgenic construct- Symbol
- Expressed Components
- None
- Knock-down Targets
- None
- Regulatory Regions