Hand1^{tm2.1(Hand1)Abfi}

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Species

Mus musculus

Symbol

Hand1^{tm2.1(Hand1)Abfi}

Category

allele

Allele of gene

Hand1

Transgenic Constructs

None

Synonyms

• Hand1^deltaNeo
• Hand1^{tm1.1(Hand1)Abfi}

Description

The coding region of Hand1 from a 600-bp cDNA initiating at the ATG and terminating at the stop codon followed by an EGFP expression cassette and a PGKNeo cassette flanked by loxP sites were inserted in frame at the position of the initiation codon. The PGKNeo cassette from Hand1^{tm2(Hand1)Abfi} was removed via cre-mediated recombination. EGFP expression is not detected in E9.5 embryos. qRT-PCR indicates that heterozygotes express about 50% of wild-type mRNA levels while homozygotes express at 30% that of wild-type, indicating that this is a hypomorphic allele. In situ analysis indicates that expression is not uniformly reduced throughout the embryo.

Additional Information

Literature

Transgenic Constructs

Genomic Variant Information

Variant Molecular Consequences

Phenotypes

Disease Associations