Evx2/Hoxd13^{tm5.1(Hoxd11/lacZ)Ddu}

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Species

Mus musculus

Symbol

Evx2/Hoxd13^{tm5.1(Hoxd11/lacZ)Ddu}

Category

allele

Allele of gene

Evx2

Transgenic Constructs

None

Synonyms

• Evx2Hoxd13^{Tg(hoxd11lac)Ddu}
• HoxD^TgHd11lacNsi

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Description

The E. coli lacZ gene was inserted, with translational reading frames aligned, into the first exon of the mouse Hoxd11 gene in a construct containing the entire Hoxd11 transcription unit and its known cis-acting 5'and 3' regulatory sequences. A construct containing this fusion gene followed by an oppositely oriented loxP-flanked PGK-neo selection cassette was targeted to the intergenic region between the transcription initiation sites of Evx2 and Hoxd13, at the 5' extremity of the Hoxd complex, with Hoxd11/lacZ in the same transcriptional orientation as Hoxd13. The selection cassette was subsequently deleted by Cre recombinase-mediated recombination, leaving a single loxP site downstream/3' of the Hoxd/lacZ fusion gene.

Additional Information

Literature

Transgenic Constructs

Genomic Variant Information

Variant Molecular Consequences

Phenotypes

Disease Associations