Mbd3^tm2Bh

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Species

Mus musculus

Symbol

Mbd3^tm2Bh

Category

allele

Allele of gene

Mbd3

Transgenic Constructs

None

Synonyms

• Mbd3^2lox
• mbd3^f

Show All 4

Description

This allele, in which exon 1 is flanked by loxP sites, was generated in embryonic stem (ES) cells heterozygous for Mbd3^tm1Bh using a negative selection approach. First, a loxP-flanked hygromycin/thymidine kinase (hyg/tk) cassette was introduced into the wild-type allele upstream of exon 1. The ES cells were re-targeted with a vector having loxP sites flanking exon 1; two rounds of exposure to 6-thioguanine selected for cells in which the hyg/tk-tagged exon 1 had been replaced by the floxed exon. Immunoblot analysis of nuclear extracts of ES cells heterozygous for this floxed allele and the null Mbd3^tm1Bh allele using antibody to MBD3 demonstrated expression of both normal protein isoforms, one of which (referred to as Mbda) contains the entire methyl-CpG-binding domain (MBD) and the other (Mbdb) lacking the N-terminal half of the MBD, encoded by exon 1. Following Cre recombinase-mediated excision of exon 1, neither isoform is detected.

Additional Information

Literature

Transgenic Constructs

Genomic Variant Information

Variant Molecular Consequences

Phenotypes

Disease Associations