Tg(CAG-XBP1*/Venus)#Miur

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Species

Mus musculus

Symbol

Tg(CAG-XBP1*/Venus)#Miur

Category

allele

Allele of gene

None

Transgenic Constructs

None

Synonyms

• ERAI
• XBP1/deltaDBD-venus

Description

Nucleotides 410-633 of a human XBP1 cDNA including a Kozak sequence and FLAG-tag coding sequence was fused to the venus fluorescent reporter and then placed under the control of the CAG promoter construct in the pCAX expression vector (pCAX is a variant of pCAGG). The XBP1 fragment lacks the DNA binding domain but includes a 26-nt endoplasmic reticulum stress specific intron. Under normal conditions, the mRNA of the fusion gene is not spliced and translation terminates at a stop codon near the junction of XBP1 and venus. During ER stress, the 26 nt intron is spliced out and translation terminates at the stop codon of venus.

Additional Information

Literature

Transgenic Constructs

Genomic Variant Information

Variant Molecular Consequences

Phenotypes

Disease Associations