Recombinase-mediated cassette exchange (RMCE) replaced the expression cassette between the JT15 and lox2272 sites of H2-T3tm1Maoh with (5' to 3') an IRES, lacZ, polyadenylation sequence, CAG promoter, hygro gene, polyadenylation sequence, FRT site, polyadenylation sequence, and CAG driven EGFP. Flp-mediated recombination removes all but the CAG promoter EGFP reporter construct flanked by an FRT site and the lox2272 site.