Atad2b^{Tg(Tyr)2295B-4Ove}

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Species

Mus musculus

Symbol

Atad2b^{Tg(Tyr)2295B-4Ove}

Category

allele

Allele of gene

Atad2b

Transgenic Constructs

None

Synonyms

• OVE#2295B-4
• OVE2295B-4

Description

A lentiviral transgenic approach was used to generate these mice. The Tyro-WPRE-FUGW lentiviral transgene (LV2177) was designed with a mouse tyrosinase minigene (Tyro) and woodchuck hepatitis virus post-transcriptional regulatory element (WPRE) in the FUGW self-inactivating HIV-based lentiviral vector backbone. The Tyro minigene replaced the ubiquitin-c promoter and EGFP sequences originally found in the FUGW lentiviral vector. The Tyro minigene is composed of the mouse Tyr enhancer region (623 bp), promoter region (657 bp), and 1566 bp cDNA sequence (including the stop codon); all in sense orientation relative to the FUGW backbone. There is no polyA site between the Tyro minigene and WPRE sequence. The WPRE sequence functions to enhance the mRNA transcript stability. In line OVE2295B-4, the lentivirus integrates into intron 9 [NCBI37/mm9; 3'-4,960,465(+)] in the sense orientation.

Additional Information

Literature

Transgenic Constructs

Genomic Variant Information

Variant Molecular Consequences

Phenotypes

Disease Associations