Version: 8.0.0Date: Tue Jan 28 2025
Allele/Variant
Tg(Dmp1-cre/ERT2)D77Pdp
- Species
- Mus musculus
- Symbol
- Tg(Dmp1-cre/ERT2)D77Pdp
- Category
- allele
- Allele of gene
- None
- Transgenic Constructs
- Synonyms
- Dmp1-CreERt2
- Description
- A fragment (-9624 - +4439) of the Dmp1 gene containing a 9624 bp promoter region, the 95 bp exon 1, 4326 bp intron 1 and 17 bp of the initial non-coding region of exon 2 was cloned into a vector in front of the cre/ERT2 cDNA. The linearized vector was injected into B6C3F1 pronuclei. 14 pups that were positive for cre integration were identified and six independent founders were crossed to wild-type B6 mice. Two founders demonstrated penetrant excision of the STOP cassette when crossed to Rosa26-lacZ reporter mice, and were analyzed in greater detail. Two 10kb-Dmp1-Cre-ERT2 transgenic founder lines (D77 and 0022) were identified to have tamoxifen-inducible Cre recombinase activity in both osteocytes and mature osteoblasts. The two lines were similar with the only difference that line D77 had greater expression in mature osteoblasts.
- Additional Information
- Literature
Transgenic Constructs
This allele contains 1 transgenic construct- Symbol
- Expressed Components
- None
- Knock-down Targets
- None
- Regulatory Regions
- Dmp1