ENU induced a T to C transition at position 15196 in the Genbank genomic region NC_000078 for the Zbtb1 gene on Chromosome 12 (77.471-77.498 Mb (+)). The mutation corresponds to nucleotide 701 of the Zbtb1 transcript in exon 2 of 2 total exons. The mutation results in a cysteine to arginine substitution at amino acid 74 of the ZBTB1 protein.