Tg(Pklr-HRAS*G12V)23Ak

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Species

Mus musculus

Symbol

Tg(Pklr-HRAS*G12V)23Ak

Category

allele

Allele of gene

None

Transgenic Constructs

None

Synonyms

• L-PK/H-ras

Description

This transgene includes a 4.9-kb BamHI fragment containing the 4 exons of the human HRAS gene with a single amino acid substitution of a glycine to valine at codon 12 under the control of the regulatory regions of the rat L-type pyruvate kinase (1.46 kb of the 5' flanking sequence). In this construct, the distal activator sequence of Pklr located around position -3 kb is removed. The single amino-acid substitution produces a constitutively active product. The Pklr regulatory sequences confer positive transcriptional control by glucose so maximal expression of the transgene can be obtained under a carbohydrate-rich diet.Three lines were made, lines 11, 21, and 23. Twenty copies of the transgene were inserted into the genome in line 23 and Northern blot confirmed expression in small intestine and low levels in the liver.

Additional Information

Literature

Transgenic Constructs

Genomic Variant Information

Variant Molecular Consequences

Phenotypes

Disease Associations