Cacna1a^tm2.1Kewa

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Species

Mus musculus

Symbol

Cacna1a^tm2.1Kewa

Category

allele

Allele of gene

Cacna1a

Transgenic Constructs

None

Synonyms

• MPI-118Q

Description

A floxed neo cassette was inserted upstream of exon 46 and exon 47 was replaced with a modified human exon 47 containing 114 CAG repeats interrupted by 4 CAA units in multiple places. A splice site mutation was inserted flanking the repeat region preventing production of the MPc and MPII isoforms. Cre meditated recombination removed the floxed neo cassette. Quantitative RT-PCR detected similar levels of expression of transcript in the cerebellum of homozygous mutant and wild-type mice. Sequencing of cDNAs from cerebellar extracts confirmed the absence of the MPc and MPII isoforms in homozygous mice.

Additional Information

Literature

Transgenic Constructs

Genomic Variant Information

Variant Molecular Consequences

Phenotypes

Disease Associations