Chat^tm2(cre)Lowl

---

Species

Mus musculus

Symbol

Chat^tm2(cre)Lowl

Category

allele

Allele of gene

Chat

Transgenic Constructs

Synonyms

• ChAT-IRES-Cre

Description

An optimized internal ribosome entry sequence (IRES) fused to cre recombinase followed by a neomycin resistance cassette flanked FRT sites was inserted after the ChAT stop codon in BAC (bMQ185k21) using homologous recombination in bacteria. A genomic ChAT fragment with 4kb homology arms on either side of the IRES-Cre insertion site was lifted out from the BAC using reverse recombineering techniques and transformed into ES cells. This allele was used to generate Chat^tm1(cre)Lowl.

Additional Information

Literature

Transgenic Constructs

Genomic Variant Information

Variant Molecular Consequences

Phenotypes

Disease Associations