Mlana^tm1.2Bee

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Species

Mus musculus

Symbol

Mlana^tm1.2Bee

Category

allele

Allele of gene

Mlana

Transgenic Constructs

None

Synonyms

• MART-1^-
• Mlana^ko

Description

The targeting construct introduced loxP sites flanking the second and third exons and a frt-flanked neomycin resistance cassette (Pgk-neo) immediately upstream of the first loxP site, in the first intron. Mice bearing the resultant Mlana^tm1Bee allele were crossed sequentially to FLP and Cre recombinase-expressing mice to delete the Pgk-neo cassette (producing Mlana^tm1.1Bee) and the loxP-flanked exons, respectively. The deleted exons 2, containing the translation initiating ATG, and 3 together encode the transmembrane domain of the protein. Reverse transcription-polymerase chain reaction (RT-PCR) analysis demonstrated the absence of Mlana transcripts in skin of mice homozygous for the present mutation. No MLANA protein was detected either by western blot analysis of extracts of melanocytes cultured from the mutant mice or by immunohistologic analysis of these melanocytes or of skin sections using antibodies to the protein's C-terminus.

Additional Information

Literature

Transgenic Constructs

Genomic Variant Information

Variant Molecular Consequences

Phenotypes

Disease Associations