Pvalb^{tm4.1(flpo)Hze}

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Species

Mus musculus

Symbol

Pvalb^{tm4.1(flpo)Hze}

Category

allele

Allele of gene

Pvalb

Transgenic Constructs

Synonyms

• Pvalb-2A-Flpo
• Pvalb-2A-FlpO-deltaHygro

Description

ES cells already targeted with a T2A-Cre vector immediately downstream of the parvalbumin translational STOP codon, were re-targeted with a "T2A-Flpo" vector and a FLP-expressing plasmid to facilitate recombination. Correctly targeted ES cells had (from 5' to 3') a three amino acid linker sequence, an frt3 site within Pvalb intron 3, a partial Pvalb exon 4 sequence up to (but not including) the endogenous stop codon, a T2A sequence in-frame with the Pvalb coding sequence, a FlpO recombinase gene (a codon-optimized FLPe modified for translation in mammalian cells and higher recombination efficiency), the Pvalb 3' UTR sequence from exon 4, an attB site, a PGK-hygromycin-SV40polyA cassette (with a frt5 site in the hygromycin gene), and an attP site. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were bred to PhiC31-expressing mice (C57BL/6J congenic background; see JAX Stock No. 007743) to delete the attB/attP-flanked PGK-hygromycin-polyA cassette. Heterozygous mice have an FlpO recombinase expression pattern in the brain similar to endogenous Pvalb expression.

Additional Information

Literature

Transgenic Constructs

Genomic Variant Information

Variant Molecular Consequences

Phenotypes

Disease Associations