Allele/Variant

Braftm1.1Brd

Species
Mus musculus
Symbol
Braftm1.1Brd
Category
allele
Allele of gene
Braf
Transgenic Constructs
None
Synonyms
  • BrafV637E
Description
A lox2272 flanked polyadenylation signal was further enclosed in "Sleeping Beauty" inverted terminal repeats. This structure was inserted in intron 17 and followed by an FRT flanked puromycin resistance cassette. In addition, a T1910A point mutation modified codon 637 in exon 18 so that it encoded glutamic acid rather than valine (p.V637E), recapitulating the human p.V600E mutation. Properly targeted males were mated to females ubiquitously expressing flp recombinase in order to remove the selection cassette. The remaining STOP sequence prevents allele expression but can be removed by cre expression.
Additional Information
Literature

Transgenic Constructs

No data available

Genomic Variant Information

No data available

Variant Molecular Consequences

Phenotypes

Disease Associations