This transgene construct contains a 2.25 kb murine Mut cDNA under the control of an alpha-fetoprotein enhancer and murine minimal albumin promoter cloned between two full-length tandem repeated chromatin barrier [hypersensitive site 4 from the chicken beta-globin cluster locus control region (cHS4)] insulator elements. Three founder lines were established. This represents the lowest expressing line. The pound symbol (#) is used because the line number is not specified.