Hprt1^{tm337a(Ple285-icre/ERT2)Ems}

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Species

Mus musculus

Symbol

Hprt1^{tm337a(Ple285-icre/ERT2)Ems}

Category

allele

Allele of gene

Hprt1

Transgenic Constructs

Synonyms

• Ple285-icre/ERT2;mEMS5786
• POU4F2-creERT2

Description

The transgene consists of a RP11-72.H2 human bacterial artificial chromosome (BAC) retrofitted to contain a human HPRT complementary sequence (containing exon 1, intron 1, exon 2 and part of intron 2), a mouse 3' Hprt homology arm, an I-Sce linearization cut site, and a mouse 5' Hprt homology arm; all inserted into the sacB gene in the BAC vector. Next, an iCre/ERT2 fusion gene, an intron, an SV40 early polyA signal and a 48-bp full Frt (Ffrt) site-flanked kanamycin resistance (Kan) cassette were inserted directly at the ATG of the human POU4F2 locus. Transient transfection with a FLP recombinase plasmid removed the Kan cassette before the modified BAC was used for targeting as a single copy "knock-in" into the upstream region of the Hprt locus.

Additional Information

Literature

Transgenic Constructs

Genomic Variant Information

Variant Molecular Consequences

Phenotypes

Disease Associations