Version: 8.0.0Date: Tue Jan 28 2025
Allele/Variant
Gt(ROSA)26Sortm95.1(CAG-GCaMP6f)Hze
- Species
- Mus musculus
- Symbol
- Gt(ROSA)26Sortm95.1(CAG-GCaMP6f)Hze
- Category
- allele
- Allele of gene
- Gt(ROSA)26Sor
- Transgenic Constructs
- None
- Synonyms
- Ai95(RCL-GCaMP6f)
- Ai95D
- Description
- The targeting vector was designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (from pCAGGS), a loxP-flanked STOP cassette (stop codons in all three reading frames linked to synthetic pA-hGHpA-PGKpA), the GCaMP6f coding sequence, a WPRE sequence (to enhance mRNA transcript stability), a bovine growth hormone polyA signal, the GCaMP6f coding sequence (described below), a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance mRNA transcript stability), a bovine growth hormone polyA signal, an AttB site, a PGK promoter-Neomycin resistance gene-PGK polyA cassette, and an AttP site. This entire targeting vector was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus. GCaMP6f has a chicken smooth muscle M13 fragment of myosin light chain kinase, a circularly permutated EGFP, and a rat calmodulin DNA fragment.
- Additional Information
- Literature