Cyp4f18^tm1.1Pchr

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Species

Mus musculus

Symbol

Cyp4f18^tm1.1Pchr

Category

allele

Allele of gene

Cyp4f18

Transgenic Constructs

None

Synonyms

• Cyp4f18 knockout
• Cyp4f18^-

Description

Flp recombinase expression was used to excise an FRT flanked neo cassette. Subsequent expression of cre removed the loxP flanked exons 8 and 9. Real time PCR analysis confirmed loss of exon 8 and 9 expression in homozygous mutant mice. The mutant RNA transcript codes for a truncated translation product that lacks the C-terminal 219 amino acids, because deletion of exons 8 and 9 results in a frameshift mutation and introduces a premature stop codon 40 bp downstream of the start of exon 10. Western blot analysis of membrane protein extracts from mutant neutrophils using an antibody that reacts with amino acids 353-370 confirmed loss of expression of this critical C-terminal region.

Additional Information

Literature

Transgenic Constructs

Genomic Variant Information

Variant Molecular Consequences

Phenotypes

Disease Associations