This transgene consists of exon 1 of the human HTT with the expansion of 84 CAG trinucleotide repeats, which encodes 153 amino acids, in the 3' end of the GFP gene, where the stop codon was removed and mutant HTT was ligated in frame, under the control of the human polyubiquitin-C promoter. Western blotting confirmed protein expression in the heart, liver, lung, spleen, kidney, muscle, and brain, with highest levels in the brain. GFP expression was confirmed in the brain.