The transgene contains the cardiac-specific mouse promoter, IRES, translation initiation sequence, loxP site, lacZ reporter gene with a STOP codon and polyadenylation sequence, loxP site, enteroviral protease 2a cleavage site, enteroviral protease 2a coding sequence and polyadenylation sequence. The pound symbol (#) is used when no line is specified and/or lines are pooled. Cre-mediated recombination is required to achieve expression of the protease.