Allele/Variant

Tg(Prnp-Immt/SOD1*G93A)7Gmnf

Species
Mus musculus
Symbol
Tg(Prnp-Immt/SOD1*G93A)7Gmnf
Category
allele
Allele of gene
None
Transgenic Constructs
Synonyms
  • mito-G93ASOD1
  • mito-SOD1G93A
Description
The mito-G93ASOD1 fusion protein contains a mouse mitofilin (Immt) cDNA sequence encoding the first 187 amino acids (including the mitochondrial targeting presequence, MMP cleavage site and transmembrane domain) fused in-frame to the N-terminus of a human Cu,Zn superoxide dismutase cDNA sequence harboring the G93A mutation (SOD1) associated with amyotrophic lateral sclerosis (ALS). The SOD1 start codon was removed and substituted with an in-frame 6 nt DNA linker. This mito-G93ASOD1 sequence was inserted between exon 2 and exon 3 of mouse prion protein (PrP or Prnp) gene at a unique XhoI site in the MoPrP.Xho plasmid vector. The founder line with the highest expression level (founder line 7) was characterized.
Additional Information
Literature

Transgenic Constructs

This allele contains 1 transgenic construct
Symbol
Expressed Components
Knock-down Targets
None
Regulatory Regions
None
All alleles with this construct

Genomic Variant Information

No data available

Variant Molecular Consequences

Phenotypes

Disease Associations