The transgene was designed with a human cytomegalovirus immediate early promoter enhancer with chicken beta-actin/rabbit beta-globin hybrid promoter (CAG; from the pCAGGS vector) driving expression of a fusion of a red fluorescent protein (RFP) with a Strep-tag sequence to facilitate isolation, an enhanced green fluorescent protein (EGFP), and a microtubule-associated protein 1 light chain 3 beta (Map1lc3b or LC3) gene.