The lentiviral gene trap vector sinLV-pRRL-K contains a gene trapping splice acceptor; stop codons in all three reading frames; an internal ribosomal entry site (IRES); the Venus reporter gene with a nuclear localization signal; a woodchuck hepatitis post-transcriptional regulatory element (WPRE); central poly-purine tract (cPPT); Rev-responsive element (RRE); and Rous sarcoma virus promoter (RSV) all flanked by a self-inactivating lentiviral long terminal (LTR) consisting of U5, R and truncated U3 element (delta U3). The gene trap vector inserted into intron 9.