Vcam1^tm2Dmil

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Species

Mus musculus

Symbol

Vcam1^tm2Dmil

Category

allele

Allele of gene

Vcam1

Transgenic Constructs

None

Synonyms

• Vcam^kB

Description

Sequential tag and exchange gene targeting was used to introduce a 6 nucleotide mutation in the 5NF-B binding site of the transcriptional promoter, and a 3 nucleotide mutation in the 5UTR of Vcam1 in exon 1. The first, "tag", targeting vector replaces exon 1 (encoding the signal peptide) and part of exon 2 (encoding immunoglobulin-like domain 1) with a pgk-neomycin/TK cassettes. The Tag construct is transfected into embryonic stems cells and a single correctly targeted clone is transfected with the second construct. The second "Exchange" targeting construct replaces the neo cassette and introduces a 6 nucleotide substitution 5' of the transcription start site resulting in the disruption of the 5'NFKB1 (NF-kappaB) binding site domain and a 3 nucleotide substitution downstream of the translation start site in the 5' UTR of exon 1. The 3 nt substitution is used to distinguish the mutant and wild-type mRNA and hnRNA. Western blot ananlysis demonstrates the presence of mutant protein in heart endothelial cells.

Additional Information

Literature

Transgenic Constructs

Genomic Variant Information

Variant Molecular Consequences

Phenotypes

Disease Associations