Allele/Variant

TgTn(sb-lacZ,GFP)1Tcb

Species
Mus musculus
Symbol
TgTn(sb-lacZ,GFP)1Tcb
Category
allele
Allele of gene
None
Transgenic Constructs
None
Synonyms
  • SBtson
  • TgTn(sb-Trans-SA-IRESLacZ-CAG-GFP-SD:Neo)1Tcb
Description
A concatemer of sleeping beauty transposable elements each consisting of a floxed PGK-neo cassette followed by inverted repeat direct repeat sequence specific for sleeping beauty transposase flanking human BCL2 splice acceptor sequence, IRES, lacZ, poly A signal, a chimeric CAG promoter containing sequence from the chicken ACTB promoter, GFP, and splice donor sequence. Expression of GFP is not seen in the concatemer. Transposition inside a gene should result in ubiquitous expression of GFP by the CAG promoter and expression of lacZ driven by the endogenous promoter. Transposition by sleeping beauty transposase does not move the PGK-neo cassette. The concatemer inserts into chromosome 10.
Additional Information
Literature

Transgenic Constructs

No data available

Genomic Variant Information

No data available

Variant Molecular Consequences

Phenotypes

Disease Associations