Cre-mediated excision of the parental Lcn10tm1(KOMP)Vlcg allele resulted in the removal of the neomycin selection cassette and critical exon(s) leaving behind the inserted lacZ reporter sequence. Western blot analysis confirmed the absence of protein expression in heart tissue from homozygous mutant mice. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml.