Col1a1^{tm18(tetO-GFP,-cas9*)Slowe}

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Species

Mus musculus

Symbol

Col1a1^{tm18(tetO-GFP,-cas9*)Slowe}

Category

allele

Allele of gene

Col1a1

Transgenic Constructs

None

Synonyms

• Col1a1^{tm18(RNU6-gRNA:Igs18,tetO-GFP,-cas9*)Slowe}

Description

RMCE on KH2 cells inserted into the 3'UTR a RNU6 promoter driving expression of a single guide RNA targeting a nongenic region on mouse chromosome 8 followed by a tetracycline response element promoter driving inducible expression of a GFP report gene, IRES sequence, human codon optimized CRISPR-associated endonuclease cas9 (derived from Streptococcus pyogenes CRISPR associated protein 9 [SpCas9]) and polyadenylation sequence.

Additional Information

Literature

Transgenic Constructs

Genomic Variant Information

Variant Molecular Consequences

Phenotypes

Disease Associations