Version: 8.0.0
Date: Tue Jan 28 2025
Col1a1tm19(tetO-GFP,-cas9*)Slowe
MGI:5755842
Allele of Col1a1
Mus musculus
SummaryTransgenic ConstructsGenomic Variant InformationVariant Molecular ConsequencesPhenotypesDisease Associations
Allele/Variant

Col1a1tm19(tetO-GFP,-cas9*)Slowe

Species
Mus musculus
Symbol
Col1a1tm19(tetO-GFP,-cas9*)Slowe
Category
allele
Allele of gene
Col1a1
Transgenic Constructs
None
Synonyms
  • Col1a1tm19(RNU6-gRNA:Apc,-gRNA:Trp53,tetO-GFP,-cas9*)Slowe
Description
RMCE on KH2 cells inserted into the 3'UTR a RNU6 promoter driving expression of tandem guide RNAs targeting murine Apc 12 bp immediately upstream of the NGG protoscpacer-adjacent motif (PAM) sequence and Trp53 followed by a tetracycline response element promoter driving inducible expression of a GFP report gene, IRES sequence, human codon optimized CRISPR-associated endonuclease cas9 (derived from Streptococcus pyogenes CRISPR associated protein 9 [SpCas9]) and polyadenylation sequence.
Additional Information
Literature

Transgenic Constructs

No data available

Genomic Variant Information

No data available

Variant Molecular Consequences

No data available

Phenotypes

Phenotype
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    Disease Associations

    Association
    Disease Qualifier
    Disease
    Annotation details
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