A mutation in the C terminus of intron 8, c.1207-1G>A, was knocked into the locus along with a floxed neo cassette after exon 9 that was subsequently removed via cre-mediated recombination. This is one of the reported Pseudohypoaldosteronism type II-causing mutations in this gene in humans. RT-PCR and Western blot analysis indicate absence of any truncated forms which would be expected with loss of exon 9 during splicing, indicating a knock-out allele.