Uhrf2^em1Yoz

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Species

Mus musculus

Symbol

Uhrf2^em1Yoz

Category

allele

Allele of gene

Uhrf2

Transgenic Constructs

None

Synonyms

None

Description

Two sgRNAs (5'-GGTCCGCACCATCGACGGCTCC-3' and 5'-GGAGCTGCGCGAGCGGGTCT-3') were used to target the start of the coding region of exon 1 with CRISPR/Cas9. A number of different deletions were found, with this allele, number 10, representing a 277 bp deletion removing part of the 5' UTR and the start of the coding region (-180 to +97). The absence of transcripts from this allele was confirmed by qPCR and the absence of peptides by Western blot analysis of thymus and brain samples.

Additional Information

Literature

Transgenic Constructs

Genomic Variant Information

Variant Molecular Consequences

Phenotypes

Disease Associations