Using recombineering methods, the CreTM-FrtNeoFrt cassette was inserted upstream of the ATG codon in exon 2 of the Dclk1 gene in the mouse BAC clone RP23-283D6. The modified BAC construct was injected into fertilized CBA x C57BL/6J oocytes. Four founders positive for the transgene were obtained and backcrossed to C57BL/6J mice. The pound symbol (#) is used when no line is specified and/or lines are pooled.