Allele/Variant

Hes7tm3.1Ybes

Species
Mus musculus
Symbol
Hes7tm3.1Ybes
Category
allele
Allele of gene
Hes7
Transgenic Constructs
None
Synonyms
  • Hes720k
Description
A lox71 site, a neomycin resistance gene cassette and a lox2272 site were inserted into the 3' UTR in exon 4 immediately after the stop codon. Successfully targeted ES cells were transfected with a cre-expressing vector and a vector containing part of intron 74 of the human dystrophin (DMD) gene. This vector was constructed as follows: a lox66 site, genomic sequence from the last 11 bp of exon 74 up to the first 10 bp of exon 75, and a lox2272 site. The intronic sequence was shortened to 20 kb by deleting sequence from the central portion. Cre-mediated recombination recombined the genomic lox71 site with the vector lox66 site and the genomic and vector lox2272 sites with each other. This results in a knock-in allele with part of the human intron and parts of its flanking exons inserted into the 3' UTR intron of the host gene.
Additional Information
Literature

Transgenic Constructs

No data available

Genomic Variant Information

No data available

Variant Molecular Consequences

Phenotypes

Disease Associations