ENU-induced A to T transversion at base pair 66,859,488 (v38) on chromosome 18, or base pair 1,000 in the GenBank genomic region NC_000084 for the gene. The mutation corresponds to residue 1000 in the mRNA sequence NM_016977 within exon 1 of 1 total exons. The mutation results in an isoleucine (I) to phenylalanine (F) substitution at position 185 (I185F) in the protein.