Prokr2^em1(icre)Cfe

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Species

Mus musculus

Symbol

Prokr2^em1(icre)Cfe

Category

allele

Allele of gene

Prokr2

Transgenic Constructs

Synonyms

• Prokr2-Cre

Description

Using CRISPR/Cas9 technology, a GSG linker, a T2A viral peptide, an iCre recombinase, and a bovine growth hormone polyadenylation signal (bPA) were inserted between the final Prokr2 codon and termination codon. The sgRNA target was cloned into the plasmid pX330. The circular pX330 plasmidand circular donor plasmid were mixed and injected into the pronuclei of B6SJLF1 fertilized zygotes. Embryos were transferred to pseudopregnant B6D2F1 females to establish the mouse line.

Additional Information

Literature

Transgenic Constructs

Genomic Variant Information

Variant Molecular Consequences

Phenotypes

Disease Associations