Allele/Variant

Zswim6tm1.2Itl

Species
Mus musculus
Symbol
Zswim6tm1.2Itl
Category
allele
Allele of gene
Zswim6
Transgenic Constructs
None
Synonyms
None
Description
A FRT-LoxP-Neomycin-FRT-LoxP selection cassette was inserted downstream of exon 4 and the third single LoxP site was inserted upstream of exon 4. The region flanked by the second and third LoxP sites is approximately 569 bp. Exon 4 encodes amino acids 337-386 and deletion of this sequence results in the creation of a premature stop codon. The neomycin selection cassette was removed via FLP-mediated recombination, leaving loxP sites flanking exon 4. Exon 4 was susequently removed via Cre-mediated recombination. In situ hybridization and RT-PCR analysis confirmed that this allele lacks expression of exon 4. qPCR analysis revealed that the medial ganglionic eminence of homozygous mutants expresses transcript downstream of the deleted region at ~35% of control levels at E13.5.
Additional Information
Literature

Transgenic Constructs

No data available

Genomic Variant Information

No data available

Variant Molecular Consequences

No data available

Phenotypes

Phenotype
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    Disease Associations

    Association
    Disease Qualifier
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