Version: 8.0.0Date: Tue Jan 28 2025
Allele/Variant
Oxtrtm1.1(cre)Hze
- Species
- Mus musculus
- Symbol
- Oxtrtm1.1(cre)Hze
- Category
- allele
- Allele of gene
- Oxtr
- Transgenic Constructs
- Synonyms
- Oxtr-T2A-Cre-D
- Description
- The targeting vector contained, from 5' to 3', a partial Oxtr sequence spanning intron 1 up to but not including the endogenous stop codon in exon 2 (with a frt3 site in intron 1), a viral 2A oligopeptide sequence (T2A; mediates ribosomal skipping), a Cre recombinase gene, a bovine growth hormone polyA sequence, an AttB site, a PGK/gb2 promoter-Neomycin resistance gene-PGK polyA cassette, a frt5 site, an mRNA splice acceptor sequence, the 3' portion of the hygromycin gene (Hygro2) with SV40 polyA signal, and an AttP site. PhiC31-mediated recombination removed the AttB/AttP-flanked sequences (PGK/gb2-Neo-pA::frt5::RNA splice acceptor::3'hygro-pA) and replace it with the recombined AttB/AttP site (AttL).
- Additional Information
- Literature
Transgenic Constructs
This allele contains 1 transgenic construct- Symbol
- Expressed Components
- None
- Knock-down Targets
- None
- Regulatory Regions