ENU-induced T to C transition at base pair 14,884,116 (v38) on chromosome 1, or base pair 34,899 in the GenBank genomic region NC_000067 encoding Trpa1. The mutation corresponds to residue 2,585 in the mRNA sequence NM_177781 within exon 21 of 27 total exons. The mutation results in a leucine (L) to proline (P) substitution at position 853 (L853P) in the TRPA1 protein.