Polr3a^tm1.1Bebr

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Species

Mus musculus

Symbol

Polr3a^tm1.1Bebr

Category

allele

Allele of gene

Polr3a

Transgenic Constructs

None

Synonyms

• Polr3a^G672E
• Polr3a^KI

Description

A lox2272 site was inserted in forward orientation into intron 14, approximately 100 bp upstream of the exon 15 splice acceptor. A FRT-flanked neomycin cassette was inserted into intron 15, approximately 150 bp downstream of the splice donor. The neo cassette was followed by a loxP site in forward orientation. The mutant exon 15 containing a G to A change at position 2015, resulting in a glycine to glutamate mutation at amino acid 672, was inserted in reverse orientation 5' to the loxP site. The inverted exon was followed by a FLEX switch consisting of lox2272 and loxP in reverse orientation. Cre-mediated recombination resulted in inversion of the mutant exon 15 so that it is expressed.

Additional Information

Literature

Transgenic Constructs

Genomic Variant Information

Variant Molecular Consequences

Phenotypes

Disease Associations