Version: 8.0.0Date: Tue Jan 28 2025
Allele/Variant
Tg(ACTB-EGFP,-Kcnj8*Q53R*G343D)1Nich
- Species
- Mus musculus
- Symbol
- Tg(ACTB-EGFP,-Kcnj8*Q53R*G343D)1Nich
- Category
- allele
- Allele of gene
- None
- Transgenic Constructs
- Synonyms
- None
- Description
- This transgene consists of the rat Kcnj8 cDNA with a glutamine to arginine substation at amino acid 53 (Gly343Asp) and a glycine to aspartate substitution at amino acid 343 (Gly343Asp), rendering ATP-insensitivity, under the control of the CX1 promoter which contains the chicken beta-actin promoter followed by the enhanced green fluorescent protein coding region (including a stop codon) flanked by loxP sites. EGFP is ubiquitously expressed unless cre-recombinase is expressed which excises the EGFP and results in expression of the mutant Kcnj8. Lines 1 and 2 are the high expressing lines, with line 1 entered as the representative line.
- Additional Information
- Literature
Transgenic Constructs
This allele contains 1 transgenic construct- Symbol
- Expressed Components
- Knock-down Targets
- None
- Regulatory Regions
- None