ENU-induced T to C transition at base pair 144,840,707 (v38) on chromosome 7, or base pair 2,751 in the GenBank genomic region NC_000073 encoding Fgf3. The mutation corresponds to residue 353 in the mRNA sequence NM_008007 within exon 2 of 3 total exons. The mutation results in a valine to alanine substitution at amino acid 86 (V86A) in the FGF3 protein.