ENU-induced T to A transversion at base pair 71,326,340 (v38) on chromosome 6, or base pair 3,529 in the GenBank genomic region NC_000072 encoding Cd8b1. The mutation corresponds to residue 467 in the mRNA sequence NM_009858 within exon 2 of 6 total exons. The mutation results in a leucine to glutamine substitution at position 133 (L133Q) in the CD8B1 protein.