Sh3yl1^tm1Syb

---

Species

Mus musculus

Symbol

Sh3yl1^tm1Syb

Category

allele

Allele of gene

Sh3yl1

Transgenic Constructs

None

Synonyms

• SH3YL1 KO

Description

The targeting vector used to create the Sh3yl1^{tm1a(KOMP)Wtsi} allele was used to create this knockout allele. The L1L2_Bact_P cassette was inserted at position 30939123 of Chromosome 12 upstream of exon 5 (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 5 at position 30939920. Exon 5 is thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml

Additional Information

Literature

Transgenic Constructs

Genomic Variant Information

Variant Molecular Consequences

Phenotypes

Disease Associations