Allele/Variant

Pole3em4Tbo

Species
Mus musculus
Symbol
Pole3em4Tbo
Category
allele
Allele of gene
Pole3
Transgenic Constructs
None
Synonyms
  • Pole3m4
Description
A 2 bp insertion (GC or CG) in the 3' end of the coding region was created using sgRNAs (targeting CGGGAGCAGAAAGGCAAG) and an ssODN template (CGGTTTGTCTAATTAAACCATAATATCCTGCTTCCTTACAGCGTACAGGCAGGAACAGAAGGGCAAGAAGGAGGCTTCGGAGCAAAAGAAGAAGGACAAAGACAAAAAGGA) with CRISPR/Cas9 technology. The resulting frameshift creates a large net positive charge on the normally negatively charged tail of the encoded peptide.
Additional Information
Literature

Transgenic Constructs

No data available

Genomic Variant Information

No data available

Variant Molecular Consequences

Phenotypes

Disease Associations