The targeting cassette introduced into intron 1 a floxed stop cassette, a recombinant nonstructural protein recognition site NS3/4A (allows for chemical inhibition with asunaprevir), self-cleaving 2A proteinase sequence, ribosomal localized EGFP reporter gene (EGFP/L10), self-cleaving 2A proteinase sequence, and an inducible kinase domain from an RNA-activated protein kinase (iPKR).