Irs4^{tm1.1(cre)Dolsn}

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Species

Mus musculus

Symbol

Irs4^{tm1.1(cre)Dolsn}

Category

allele

Allele of gene

Irs4

Transgenic Constructs

Synonyms

• IRS4-iCre

Description

Using a BAC recombineering method, a targeting construct containing an insertion of an Frt-flanked neomycin selection cassette followed by an internal ribosomal entry (tires) sequence fused to a Cre recombinase transgene (iCre) between the STOP codon and the polyadenylation of the Irs4 gene. This construct was used to isolate a targeting plasmid with the neo-iCre cassette with upstream and downstream Crh homology arms. ES cells were targeted and used for blastocyst injection. Chimeras were bred for germline transmission. The selection marker was excised by breeding to Flp deleter animals.

Additional Information

Literature

Transgenic Constructs

Genomic Variant Information

Variant Molecular Consequences

Phenotypes

Disease Associations