A targeting vector was designed to replace the entire coding region of the prepro-Qrfp sequence in exon 2 of the Qrfp gene with iCre and a pgk-Neo cassette so that the endogenous Qrfp promoter drives the expression of iCre. The Pgk-Neo cassette was deleted by crossing the mice with FLP66 mice. It is a knock-in, knock-out allele.